A main city on the island of Lesbos in the Aegean Sea near the northwestern coast of Asia Minor. Mitylene was a seaport with two harbors. They first built it on a small island separate from Lesbos.
In New Testament times, a raised roadway across a narrow stretch of water connected it with the main island. Acts 20:14 identifies Mitylene as one of the overnight stopping places where Paul and his traveling companions lodged. This was as they journeyed by ship toward Jerusalem.